Biofilms 2015: Multi-disciplinary approaches shed light into microbial life on surfaces.
Published in Journal of Bacteriology
Published in Journal of Bacteriology
Published in Molecular Microbiology
FliG and FliM are switch proteins that regulate the rotation and switching of the flagellar motor. Several assembly models for FliG and FliM have recently been proposed; however, it remains unclear whether the assembly of the switch proteins is conserved among different bacterial species. We applied a combination of pull-down, thermodynamic and str...
Published in Proteins Structure Function and Bioinformatics
An important problem in computational biology is predicting the structure of the large number of putative proteins discovered by genome sequencing projects. Fold-recognition methods attempt to solve the problem by relating the target proteins to known structures, searching for template proteins homologous to the target. Remote homologs that may hav...
Published in Journal of Comparative and Physiological Psychology
Published in Journal of Bacteriology
Vibrio cholerae, the causative agent of cholera, is a facultative human pathogen with intestinal and aquatic life cycles. The capacity of V. cholerae to recognize and respond to fluctuating parameters in its environment is critical to its survival. In many microorganisms, the second messenger, 3 ,5 -cyclic diguanylic acid (c-di-GMP), is believed to...
Published in Arthritis & Rheumatism
Collagen-induced arthritis can be transferred from immunized arthritic rats to unimmunized recipients by intravenous injection of an immunoglobulin concentrate of sera. This study identifies antibodies in the transfer concentrate which localize to the articular surface of cartilage in joints of recipients. Immunoglobulin can also be demonstrated in...
Published in Genetics
The usefulness of genomic physical maps is greatly enhanced by linkage of the physical map with the genetic map. We describe a "macrorestriction mapping" procedure for Caenorhabditis elegans that we have applied to this endeavor. High molecular weight, genomic DNA is digested with infrequently cutting restriction enzymes and size-fractionated by pu...
Published in Genome Research
The National Institutes of Health s Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues....
Published in Nautre Structural Biology
Translational release factors decipher stop codons in mRNA and activate hydrolysis of peptidyl-tRNA in the ribosome during translation termination. The mechanisms of these fundamental processes are unknown. Here we have mapped the interaction of bacterial release factor RF1 with the ribosome by directed hydroxyl radical probing. These experiments i...
Published in Immunology
Recombinant human type II collagen (rhCII) was produced using both the HT1080 mammalian cell expression system (rhCIIht) and a baculovirus expression system (rhCIIbac). The biosynthesis of CII requires extensive post-translational modifications, such as the hydroxylation of prolyl and lysyl residues and glycosylation of hydroxylysyl residues. Amino...